Electrophoresis is a method to separate different
molecules (including DNA). Gel electrophoresis separates the
different molecules in a mixture by letting them pass through a
gel, driven by an electric
current. The gel is placed in a tank with an ionic
buffer, and the mixture to separate is injected onto the gel near
anode. When the electric current is applied, the (negatively
charged) molecules travel towards the (positive)
cathode. The smaller and the more negatively charged the molecules
are, the faster they pass through the gel.
After the electrophoresis run, when the smallest molecules have
almost reached the anode, the molecules in the gel can be
stained to make them visible.
medicine, protein electrophoresis is a method of
analysing a mixture of
proteins by means of
gel electrophoresis, mainly in
plasma is not suitable).
Are you a doctor or a nurse?
Do you want to join the Doctors Lounge online medical community?
Participate in editorial activities (publish, peer review, edit) and
give a helping hand to the largest online community of patients.
Click on the link below to see the requirements:
Doctors Lounge Membership
There are two large classes of
globulin. They are generally equal in proportion, but albumin is
much smaller and lightly negatively charged, leading to an
accumulation of albumin on the electrophoretic gel. A small band
transthyretin (also named
pre-albumin). Some forms of medication or body chemicals can cause
their own band, usually small (see, however,
globulins are classified by their banding pattern (with their main